Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells

Technology that can evaluate the completeness of artificial organ analogs has been developed. Researchers at the Korea Research Institute of Biotechnology have developed a quantitative evaluation algorithm and analysis web page for long-term similarity analysis with organoids derived from human starch-based stem cells. The researchers established three types of similarity evaluation panels and algorithms through public data and next-generation base analysis (NGS)-based transcriptional analysis.

It is expected to contribute greatly to the development of new drug development platforms and cell therapy by quantitatively evaluating the completeness of differentiation of lungs, gastric organoids and myocardial cells, and similarities with human organs. By providing analysis services through web pages, researchers related to organoids and cell differentiation at home and abroad can use them in various ways.

Recently, studies on organoids and differentiated cells have been actively conducted to develop new drugs (toxicity and efficacy evaluation) and cell therapy.

Toxicity and efficacy evaluation are an important part of the new drug development stage. Drugs that have not been toxic in animal-based preclinical trials show liver, kidney, and heart toxicity in clinical trials, resulting in the suspension of clinical trials.

Organoids and cells derived from human starchy stem cells that imitate various organs in the preclinical stage are attracting attention as important alternatives to preclinical animal testing. Therefore, it is important to develop differentiation technology for producing organoids.

In addition, recently, studies on organoids have been conducted as experimental models to conduct studies on pulmonary infection pathways and human reactions of COVID-19, and microbiome-related studies.

Therefore, the development of organoids and cells derived from starchy stem cells is expected to be a successful future model for disease modeling and drug screening in the treatment development, pharmacological toxicological industry.

Cho Hyun-soo, a senior researcher, said, “This research result is to develop a technology that can measure the degree of quantitative differentiation to secure the homogeneity and reproducibility of various organoids for the development of new drug evaluation platforms and cell therapy.”

He added, “Through the newly developed quantitative evaluation system, similarity evaluation will be possible for various tissues in the future, which will greatly contribute to the development of alternative models for drug screening and stem cell therapy.”

Story Source: Materials provided by Korea Research Institute of Bioscience and Biotechnology.

Journal Reference: Lee, MO., Lee, Sg., Jung, CR. et al. Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells. Nat Commun 12, 4492 (2021).

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